ABSTRACT
Piper nigrum has been used in Indonesian traditional medicine to alleviate pain. Piperine, a nitrogenous substanceisolated from the plant, has been reported for its anti-inflammatory activity. However, this compound is slightlysoluble in water, which impacts its bioaccessibility. A recent study reported that a co-ground mixture of piperine andβ-cyclodextrin revealed a significant increase of dissolved piperine at 15 minutes of dissolution test compared to thatof pure piperine. This work was aimed to study the bioaccessibility of the carrageenan-complexed piperine in Wistarrats and assayed its anti-inflammatory activity on the edema-induced paw of the rats. Both isolated (from P. nigrum)and synthetic (TCI, Tokyo Chemical Industry) piperines were used as the standards for the bioaccessibility assay,whereas acetosal was the standard drug for the anti-inflammatory activity study. The carrageenan-complexed piperinerevealed a better bioaccessibility (Cmax = 0.34 µg/ml; Tmax at 30 minutes) than that of the isolated piperine (Cmax = 0.12µg/ml, Tmax at 60 minutes), whereas the synthetic piperine showed the best absorption (Cmax = 0.48 µg/ml, Tmax at 30minutes). The anti-inflammatory activity of carrageenan-complexed piperine at a dose of 393 mg/kg body weight(BW) (contains 100 mg of piperine) equals to the acetosal dose of 45 mg/kg BW. Thus, the inclusion of biopiperinein the carrageenan complex might improve its bioaccessibility and in vivo anti-inflammatory activity in Wistar rats.
ABSTRACT
In the last 20 years, plant-based therapy had become the world's attention. Our country Indonesia is popular in itsmegabiodiversity, among which is Salacca zalacca Gaert. (Voss.), or salak. The fruit of this plant is generally utilizedas pickles, chips, etc. However, its benefits for health remain unexplored. Library searches using PubMed, GoogleScholar, Science Direct, and Google resulted that S. zalacca, which contains flavonoids, alkaloids, terpenoids, andsitosterols exerts various pharmacology activities, e.g., antioxidant, cholesterol reducer, antidiabetic, skin whitening,antihyperuricemia, antibacterial, immune system enhancer, cancer cell growth inhibitor, and adsorbent.
ABSTRACT
Sintoc bark (Cinnamomum sintoc Bl) belongs to Lauraceae (the laurel family). It has been used empirically for a treatment for swelling caused by insects’ bites. In this study, the research examined the analgesic activity and anti-inflammation of essential oil of sintocbark using in vivo methods. The mechanism of anti-inflammation was predicted using molecular docking against COX-2. Essential oil of sintoc bark was collected by distilling through steam distillation, and then analyzed by GC-MS. Analgesic and anti-inflammatory activity was examined by in vivo, which were conducted by writhing and carrageenan-induced methods, respectively. The findings showed that the tested sintoc bark oils contained 36 components of essential oil with eugenol (38.38 %) as a major compound. In the in vivo experiments, sintoc bark oils with doses 0.005 mL, 0.010 mL, and 0.020 mL/20g body weight significantly (p<0.05) reduced the number of writhing of mice when compared to negative control group. All of doses of sintoc bark oils gave significantly affect (confidence level 99 %) compare to negative control. Sintoc oil with dose 0.2 ml/200g had the strongest inhibition compare to positive control (indometasin 10 mg/kg body weight). The molecular docking results indicated that the compounds of aryl propanoid were generally potential to inhibit COX-2.
ABSTRACT
The essential oils of three parts of the plant Alpinia malaccencis (Burm f.) (Zingiberaceae) collected from Sumedang, West Java Province has been studied. Determination of essential oil components from the rhizome, stem, and leaf of Alpinia malaccencis (Burm f.) was performed by confirmation of the linear retention index (LRI) and comparing the NIST library peak with data reported in the literature, the mass spectrum peaks with literature data. The essential oils obtained from rhizome, stem, and leaf of Alpinia malaccencis (Burm f.) by steam distillation was 1.22%, 0.25%, and 0.7%, (w/w), respectively. Rhizome oil composition was almost similar with stem oil with methyl cinnamate as the major component. Composition of leaf oil is different from rhizome and stem oil which dominated α-pinene (30.57%). The rhizome in doses 0.1 ml (47.09 %) had stronger locomotor inhibition activity compare than stem (26.97 %) and leaf oils (32.23 %) in doses 0.1 mL.